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anti crls1 antibody  (Proteintech)
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Proteintech anti crls1 antibody
Knockdown of <t>cardiolipin</t> <t>synthase</t> <t>1</t> <t>(CRLS1)</t> attenuates PANoptotic cell death, mitochondrial binding of GSDMD-NT, GSDME-NT and p-MLKL, and mitochondrial dysfunction in macrophages. (A) The expression of CRLS1 in BMDMs was knocked down with three specific siRNAs (si-CRLS1 #1, #2 and #3) in comparison with the same NC-siRNA, and Western blot analysis was used to assay CRLS1 expression in BMDMs 48 h after siRNA transfection ( (A) , left panel). The knockdown efficiency of si-CRLS1 was quantified and normalized to β-actin. Data are shown as mean ± SD ( (A) , right panel). si-CRLS1 #3 exhibited the highest knockdown efficiency and was used for the following experiments. (B) Cells were treated with OXO for 1 h, followed by stimulation with TNF-α for 2 h, mitochondrial binding of cellular PANoptosis hallmarks GSDMD-NT, GSDME-NT and p-MLKL was detected by Western blotting. COV IV was detected as an internal control for mitochondria. The values under the blots represent their relative levels. (C, D) Analysis of lytic cell death in BMDMs by propidium iodide (PI) (red, staining dying cells) staining (C) . PI-positive cells in 5 randomly chosen fields were quantified and percentage of cell death is defined as the ratio of PI-positive over all cells (revealed by Hoechst 33342) (D) . (E, F) Mitochondrial membrane potential was analyzed by staining with TMRE (E, F) . (G, H) Mitochondrial ROS generation was assayed by staining with MitoSOX (G, H) . Images were acquired by fluorescence microscopy. Histograms showing quantitative analyses. Data are shown as mean ± SD ( n = 5). Scale bars, 50 μm. ** P < 0.01; *** P < 0.001; ns, not significant.
Anti Crls1 Antibody, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti crls1 antibody/product/Proteintech
Average 93 stars, based on 1 article reviews
anti crls1 antibody - by Bioz Stars, 2026-03
93/100 stars
  Buy from Supplier
crls1  (Proteintech)
93
Proteintech crls1
Knockdown of <t>cardiolipin</t> <t>synthase</t> <t>1</t> <t>(CRLS1)</t> attenuates PANoptotic cell death, mitochondrial binding of GSDMD-NT, GSDME-NT and p-MLKL, and mitochondrial dysfunction in macrophages. (A) The expression of CRLS1 in BMDMs was knocked down with three specific siRNAs (si-CRLS1 #1, #2 and #3) in comparison with the same NC-siRNA, and Western blot analysis was used to assay CRLS1 expression in BMDMs 48 h after siRNA transfection ( (A) , left panel). The knockdown efficiency of si-CRLS1 was quantified and normalized to β-actin. Data are shown as mean ± SD ( (A) , right panel). si-CRLS1 #3 exhibited the highest knockdown efficiency and was used for the following experiments. (B) Cells were treated with OXO for 1 h, followed by stimulation with TNF-α for 2 h, mitochondrial binding of cellular PANoptosis hallmarks GSDMD-NT, GSDME-NT and p-MLKL was detected by Western blotting. COV IV was detected as an internal control for mitochondria. The values under the blots represent their relative levels. (C, D) Analysis of lytic cell death in BMDMs by propidium iodide (PI) (red, staining dying cells) staining (C) . PI-positive cells in 5 randomly chosen fields were quantified and percentage of cell death is defined as the ratio of PI-positive over all cells (revealed by Hoechst 33342) (D) . (E, F) Mitochondrial membrane potential was analyzed by staining with TMRE (E, F) . (G, H) Mitochondrial ROS generation was assayed by staining with MitoSOX (G, H) . Images were acquired by fluorescence microscopy. Histograms showing quantitative analyses. Data are shown as mean ± SD ( n = 5). Scale bars, 50 μm. ** P < 0.01; *** P < 0.001; ns, not significant.
Crls1, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/crls1/product/Proteintech
Average 93 stars, based on 1 article reviews
crls1 - by Bioz Stars, 2026-03
93/100 stars
  Buy from Supplier
anti crls1 203  (Proteintech)
93
Proteintech anti crls1 203
Knockdown of <t>cardiolipin</t> <t>synthase</t> <t>1</t> <t>(CRLS1)</t> attenuates PANoptotic cell death, mitochondrial binding of GSDMD-NT, GSDME-NT and p-MLKL, and mitochondrial dysfunction in macrophages. (A) The expression of CRLS1 in BMDMs was knocked down with three specific siRNAs (si-CRLS1 #1, #2 and #3) in comparison with the same NC-siRNA, and Western blot analysis was used to assay CRLS1 expression in BMDMs 48 h after siRNA transfection ( (A) , left panel). The knockdown efficiency of si-CRLS1 was quantified and normalized to β-actin. Data are shown as mean ± SD ( (A) , right panel). si-CRLS1 #3 exhibited the highest knockdown efficiency and was used for the following experiments. (B) Cells were treated with OXO for 1 h, followed by stimulation with TNF-α for 2 h, mitochondrial binding of cellular PANoptosis hallmarks GSDMD-NT, GSDME-NT and p-MLKL was detected by Western blotting. COV IV was detected as an internal control for mitochondria. The values under the blots represent their relative levels. (C, D) Analysis of lytic cell death in BMDMs by propidium iodide (PI) (red, staining dying cells) staining (C) . PI-positive cells in 5 randomly chosen fields were quantified and percentage of cell death is defined as the ratio of PI-positive over all cells (revealed by Hoechst 33342) (D) . (E, F) Mitochondrial membrane potential was analyzed by staining with TMRE (E, F) . (G, H) Mitochondrial ROS generation was assayed by staining with MitoSOX (G, H) . Images were acquired by fluorescence microscopy. Histograms showing quantitative analyses. Data are shown as mean ± SD ( n = 5). Scale bars, 50 μm. ** P < 0.01; *** P < 0.001; ns, not significant.
Anti Crls1 203, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti crls1 203/product/Proteintech
Average 93 stars, based on 1 article reviews
anti crls1 203 - by Bioz Stars, 2026-03
93/100 stars
  Buy from Supplier

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Knockdown of cardiolipin synthase 1 (CRLS1) attenuates PANoptotic cell death, mitochondrial binding of GSDMD-NT, GSDME-NT and p-MLKL, and mitochondrial dysfunction in macrophages. (A) The expression of CRLS1 in BMDMs was knocked down with three specific siRNAs (si-CRLS1 #1, #2 and #3) in comparison with the same NC-siRNA, and Western blot analysis was used to assay CRLS1 expression in BMDMs 48 h after siRNA transfection ( (A) , left panel). The knockdown efficiency of si-CRLS1 was quantified and normalized to β-actin. Data are shown as mean ± SD ( (A) , right panel). si-CRLS1 #3 exhibited the highest knockdown efficiency and was used for the following experiments. (B) Cells were treated with OXO for 1 h, followed by stimulation with TNF-α for 2 h, mitochondrial binding of cellular PANoptosis hallmarks GSDMD-NT, GSDME-NT and p-MLKL was detected by Western blotting. COV IV was detected as an internal control for mitochondria. The values under the blots represent their relative levels. (C, D) Analysis of lytic cell death in BMDMs by propidium iodide (PI) (red, staining dying cells) staining (C) . PI-positive cells in 5 randomly chosen fields were quantified and percentage of cell death is defined as the ratio of PI-positive over all cells (revealed by Hoechst 33342) (D) . (E, F) Mitochondrial membrane potential was analyzed by staining with TMRE (E, F) . (G, H) Mitochondrial ROS generation was assayed by staining with MitoSOX (G, H) . Images were acquired by fluorescence microscopy. Histograms showing quantitative analyses. Data are shown as mean ± SD ( n = 5). Scale bars, 50 μm. ** P < 0.01; *** P < 0.001; ns, not significant.

Journal: Frontiers in Immunology

Article Title: Anti-alcoholism drug disulfiram inhibits PANoptosis by blocking mitochondrial permeabilization in macrophages

doi: 10.3389/fimmu.2025.1726408

Figure Lengend Snippet: Knockdown of cardiolipin synthase 1 (CRLS1) attenuates PANoptotic cell death, mitochondrial binding of GSDMD-NT, GSDME-NT and p-MLKL, and mitochondrial dysfunction in macrophages. (A) The expression of CRLS1 in BMDMs was knocked down with three specific siRNAs (si-CRLS1 #1, #2 and #3) in comparison with the same NC-siRNA, and Western blot analysis was used to assay CRLS1 expression in BMDMs 48 h after siRNA transfection ( (A) , left panel). The knockdown efficiency of si-CRLS1 was quantified and normalized to β-actin. Data are shown as mean ± SD ( (A) , right panel). si-CRLS1 #3 exhibited the highest knockdown efficiency and was used for the following experiments. (B) Cells were treated with OXO for 1 h, followed by stimulation with TNF-α for 2 h, mitochondrial binding of cellular PANoptosis hallmarks GSDMD-NT, GSDME-NT and p-MLKL was detected by Western blotting. COV IV was detected as an internal control for mitochondria. The values under the blots represent their relative levels. (C, D) Analysis of lytic cell death in BMDMs by propidium iodide (PI) (red, staining dying cells) staining (C) . PI-positive cells in 5 randomly chosen fields were quantified and percentage of cell death is defined as the ratio of PI-positive over all cells (revealed by Hoechst 33342) (D) . (E, F) Mitochondrial membrane potential was analyzed by staining with TMRE (E, F) . (G, H) Mitochondrial ROS generation was assayed by staining with MitoSOX (G, H) . Images were acquired by fluorescence microscopy. Histograms showing quantitative analyses. Data are shown as mean ± SD ( n = 5). Scale bars, 50 μm. ** P < 0.01; *** P < 0.001; ns, not significant.

Article Snippet: The anti-PNPT1 antibody and anti-CRLS1 antibody (14845-1-AP) were obtained from Proteintech (Rosemont, IL, USA).

Techniques: Knockdown, Binding Assay, Expressing, Comparison, Western Blot, Transfection, Control, Staining, Membrane, Fluorescence, Microscopy